Purification of human alpha-light class lymphotoxin to electrophoretic homogeneity
نویسندگان
چکیده
منابع مشابه
Human interleukin 1. Purification to homogeneity
We have purified human interleukin 1 (IL-1) to homogeneity by a simplified procedure that results in excellent yields of pure material that retains a high level of biological activity. IL-1, secreted by human peripheral blood macrophages that have been stimulated with Staphylococcus aureus, was purified by ion exchange chromatography and affinity chromatography on Procion Red agarose. The pure ...
متن کاملPurification to Homogeneity
The protein glutaredoxin, required for GSH-dependent ribonucleotide reduction, has been purified to homogeneity from calf thymus. The preparative method consisted of ammonium sulfate precipitation and three chromatography steps on DEAEkellulose, Sephadex G-50, and CM-Sepharose. Calf thymus glutaredoxin was assayed on the basis of its inherent GSH-disulfide transhydrogenase activity. Glutaredoxi...
متن کاملElectrophoretic Homogeneity of Pregnant Mare Serum Gonadotrophin
A highly purified and potent gonadotrophin in pregnant mare serum has been prepared. The preparation has been shown to be electrophoretically homogeneous in the Tiselius apparatus. The mobilities of the substance have been determined over a wide range of hydrogen ion concentrations. The isoelectric point lies at pH 2.60-2.65 and the value of See PDF for Equation is 4.0 x 10(-5). Some chemical c...
متن کاملVASCULAR BIOLOGY Lymphotoxin-alpha contributes to lymphangiogenesis
1Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, CT; 2Department of Biomedicine, University of Bergen, Bergen, Norway; 3Department of Anesthesia and Intensive Care, Haukeland University Hospital, Bergen, Norway; 4Cardiovascular Research Institute, Comprehensive Cancer Center, and Department of Anatomy, University of California, San Francisco; 5Edwin ...
متن کاملPurification to homogeneity of Charonia lampas alpha-fucosidase by using sequential ligand-affinity chromatography.
An alpha-fucosidase from the liver of the marine gastropod Charonia lampas was purified to homogeneity using a procedure that included cation-exchange and gel-filtration chromatography, chromatofocusing and a final series of affinity-chromatography steps which involved the following gel-immobilized ligands: N-(5-carboxy-1-pentyl)-1,5-dideoxy-1,5-imino-L-fucitol, N-(5-carboxy-1-pentyl)-2-acetami...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular Immunology
سال: 1981
ISSN: 0161-5890
DOI: 10.1016/0161-5890(81)90020-1